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Hispanic Educational Technology Services

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Rapid Tolerance in Drosophila Melanogaster

Joel Osegueda Delgado

Testing for the correlation between sleep length and rapid tolerance in Drosophila melanogaster. The flies used were bred for long sleep and short sleep cycles. We saw loss of righting reflex in both trials during the alcohol sensitivity and rapid tolerance trial. This allowed us to better deduce which fly lost their righting reflex as this was more subjective making us decide a better way to decide aside from the 5 second rule if they are one their side or back. We also determined a more efficient way to collect flies as only collecting the groups we need for the experiment the next day rather than collecting as many all at once. As far as cost goes, we used materials available in the lab to create the flybar as we needed three erlenmeyer flasks, four vials to serve as administration chambers, plastic tubing, and a bubbler that is used for fish tanks. We were able to present this at a separate conference at the University of Houston-Downtown, a hispanic serving institution, where we honed our networking skills as well. Some of the lessons learned resulted in better management for our time, to be more deliberate and careful in our decisions as well as how messy research can truly be.

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